Device

Part:BBa_K2017013:Design

Designed by: Monica Victoria Gutierrez Salazar   Group: iGEM16_Valencia_UPV   (2016-10-04)


35s + TFL consense + AEK-Luciferase + Tnos


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 66
    Illegal BsaI site found at 2580
    Illegal BsaI.rc site found at 2867
    Illegal SapI.rc site found at 1726


Design Notes

It is important to notice that this device is modular. To insert the region of the gene of interest, this region must have the overhang 5'-AATG-3' and 5'-TTCG-3'.


Source

This part was obtained using GoldenBraid assembly. The TFL consense sequence was obtained with oligonucleotide synthesis.

References